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. 2016 Dec 20;10:124. doi: 10.3389/fnana.2016.00124

Figure 1.

Figure 1

Somatodendritic visualization of vasoactive intestinal polypeptide positive (VIP+) neurons. (A–A″) Specific expression of Cre mRNA in VIP-Cre knock-in mice. In the primary somatosensory cortex barrel field (S1BF) of VIP-Cre knock-in mice, 98.6 ± 0.4% (mean ± SD) of VIP-immunoreactive (VIP-ir) cells expressed Cre mRNA, and inversely, 98.2 ± 0.8% of Cre-expressing cells showed immunoreactivity for VIP. Arrowhead indicates a double-labeled neuron. Scale bar = 20 μm. (B) Construction of pAAV2-SynTetOff-FLEX-FGL. Both the myristoylation/palmitoylation site of the Fyn N-terminus (myr) and the C-terminus of the low-density lipoprotein receptor (LDLRct) were added to green fluorescent protein (GFP), referred to as FGL. FGL was previously reported to be distributed specifically on the somatodendritic membranes of neurons (Kameda et al., 2008, 2012). (C–E) Specific labeling of somatodendritic membranes of VIP+ neurons. One week after injection of AAV2/1-SynTetOff-FLEX-FGL into the S1BF of VIP-Cre knock-in mice, brain sections were double-immunostained for GFP and VIP. GFP was specifically expressed in VIP+ neurons (E; 99.0 ± 0.1% of GFP-positive cells were VIP-ir). The numerical data were acquired bilaterally from three mice (309 GFP-positive cells). Arrowheads indicate a GFP-expressing VIP+ neuron. Scale bars in (C,D) = 100 μm and 10 μm, respectively.