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. 2016 Dec 20;10:124. doi: 10.3389/fnana.2016.00124

Figure 2.

Figure 2

Somatodendritic morphology of L2/3 VIP+ neurons in the S1BF. (A) Somatodendritic morphology of VIP+ neurons in L2/3. GFP-labeled somatodendritic domains of VIP+ neurons were manually reconstructed with NeuroLucida, and all dendritic branches derived from a single VIP+ neuron were identified. Scale bar = 100 μm. (B) We defined primary dendrites with at least one branch extending in L1 as apical branches (red), and those oriented in other directions were defined as basal branches (blue). Scale bar = 100 μm. (C) Polar histogram of VIP+ neuron dendrites depicted in (B). The total dendritic length in each 10° is summed as a pie-shaped wedge. Dendrites toward the pia mater are shown at 90°, while those toward the white matter are shown at 270°. (D) Sholl analysis of VIP+ neuron dendrites depicted in (B). The number of dendrite intersections against the radial distance from the soma (every 10 μm) is plotted. Apical and basal branches are separately shown in red and blue, respectively. (E,F) Mean length of each radial bin from polar histograms of 36 VIP+ neurons shown in (A). (G) Sholl analysis of 36 VIP+ neurons. Error bars, ± SEM. †p < 0.05 using two-way ANOVA followed by Bonferroni post hoc multiple comparison test.