Table 2.

Immunofluorescence intensities for vesicular glutamate transporter 1 (VGluT1), VGluT2 and vesicular GABA transporter (VGAT) in L2/3 and L4 of the mouse primary somatosensory cortex barrel field (S1BF).

	Barrel column	Septal column	p value
	VGluT1
Upper L2/3	1.00 ± 0.04	1.04 ± 0.03	0.468
Middle L2/3	1.00 ± 0.04	1.05 ± 0.03	0.303
Lower L2/3	1.00 ± 0.03	1.03 ± 0.03	0.553
L4	1.00 ± 0.04	0.94 ± 0.02	0.244
	VGluT2
Upper L2/3	1.00 ± 0.05	1.00 ± 0.05	0.962
Middle L2/3	1.00 ± 0.04	1.00 ± 0.04	0.973
Lower L2/3	1.00 ± 0.03	1.02 ± 0.03	0.623
L4	1.00 ± 0.01	0.79 ± 0.02	<0.0001
	VGAT
Upper L2/3	1.00 ± 0.02	0.91 ± 0.10	0.382
Middle L2/3	1.00 ± 0.03	0.99 ± 0.03	0.813
Lower L2/3	1.00 ± 0.03	0.92 ± 0.10	0.451
L4	1.00 ± 0.02	0.86 ± 0.03	0.002

Mean fluorescence intensities ± SEM in the barrel and septal columns are shown. We extended the barrel and septal columns to L2/3, and measured the immunofluorescence intensities for VGluT1, VGluT2 and VGAT in the upper, middle and lower parts of L2/3 by dividing into 3 bins. Mean immunofluorescence intensities in the barrel column were standardized as 1 AU. Whereas immunofluorescence intensities for VGluT2 and VGAT were higher in barrel than in septa of L4, those in the barrel and septal columns of L2/3 were not significantly different.