Figure 5.

Systemically administered vesicular stomatitis viruses (VSV) replication is highly tumor selective with minimal infection of the liver. Immunohistochemical staining for VSV antigen (brown DAB (3,3′-Diaminobenzidine) stain) reflect robust VSV expression in (a) tumors at day 4 post-virus, and (b) minimal VSV expression in the liver of these VSV-treated mouse. (c) VSV staining of a liver section from a saline-treated animal. (d) QRT-PCR for VSV-N RNA and (e) infectious virus recovered from the liver of VSV-treated nontumor or MPC-11 bearing animals at day 2 and 4 post-virotherapy. ND, not detectable. (f) QRT-PCR showed minimal VSV replication in liver as indicated by declining VSV-N RNA in nontumor bearing Balb/c mice. Limit of detectable of VSV-N RNA by qRT-PCR is ≥1,000 copies/µg RNA, and infectious virus is >190 TCID₅₀/ml. *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001, Student's t-test.