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. 2016 Nov 15;24(12):2078–2089. doi: 10.1038/mt.2016.175

Figure 5.

Figure 5

Mouse surrogate αmuCD11a-PDE4 inhibitor ADC reduces inflammation in a mouse carrageenan air pouch model. (a) A schematic graph showing timelines for air pouch generation and drug administration. (b) αmuCD11a (M17 clone) levels in the diluted air pouch exudate and serum samples were assessed using an anti-rat IgG ELISA. (c) Exudate cytokines including TNFα, IFNγ, IL-6, IL-12p70, MCP-1, and IL-10 were assessed by a mouse inflammatory CBA assay kit. The results are presented as means ± SEM. n = 6 per group. (d) Exudate cells were stained with appropriate antibodies and subjected to flow cytometry analysis. The live cells that were CD45-positive and live/dead stain negative were designated as live CD45+ cells, within which the percentage of different cell populations were analyzed, including CD3+ T cells, CD11b+ Ly6C+ F4/80- monocytes, CD11b+ Ly6C+ F4/80+ macrophages, CD11b+ Ly6G+ Ly6Cint neutrophils. (e) Binding of αmuCD11a (M17 clone) on different exudate immune cell population was assessed by anti-rat IgG stain. (f) CD11a expression on different immune cell populations in the exudate was assessed by flow cytometry.