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. 2004 Mar 8;4:4. doi: 10.1186/1472-6807-4-4

Figure 1.

Figure 1

High-throughput domain characterisation and evaluation by 2D 1H-15N-HSQC spectroscopy. Analytical results for the SEP-domain (residues 171 – 270 from protein AAH02801.1), the N-terminal DCX domain from protein AAC31696.1 (residues 90 – 186) and the L27-domain from protein AAG34117.1 (residues 13 – 68) are shown. Samples for SDS-PAGE, analytical HIC and 1D 1H-NMR spectra were obtained from two separate automated small-scale purifications of 1 ml cultures. 2D 1H-15N-HSQC spectra resulted from the purification of a 400 ml culture. SDS-PAGE lanes: M: marker; I: NiNTA-bound protein eluted with imidazole for analytical HIC; E: NiNTA-bound protein eluted with EDTA for 1D 1H-NMR analysis.