Table 2.
Analysis of soluble constructs with 1D 1H-NMR and analytical HIC.
| Domain | Protein ID | start residue | end residue | 1H 1D-NMR | analytical HIC |
| SEP | AAH02801.1 | 171 | 270 | + | + |
| SEP | AAH02801.1 | 176 | 270 | + | + |
| DCX | AAC31696.1 | 90 | 181 | + | +/- |
| DCX | AAC31696.1 | 90 | 186 | + | + |
| DCX | AAC31696.1 | 85 | 181 | + | + |
| DCX | AAC31696.1 | 85 | 186 | + | + |
| DCX | AAC31696.1 | 212 | 310 | - | - |
| DCX | AAC31696.1 | 217 | 310 | n. d. | - |
| DCX | AAC31696.1 | 217 | 305 | n. d. | - |
| DCX | AAC31696.1 | 85 | 305 | +/- | - |
| DCX | AAC31696.1 | 90 | 305 | +/- | - |
| DCX | AAC31696.1 | 90 | 310 | - | - |
| DCX | AAC31696.1 | 85 | 310 | - | - |
| SEP-UBX | AAH02801.1 | 171 | 368 | + | + |
| SEP-UBX | AAH02801.1 | 176 | 368 | - | +/- |
| PINT | AAB58732.1 | 316 | 409 | n. d. | - |
| PINT | AAB58732.1 | 321 | 404 | n. d. | - |
| PINT | AAB58732.1 | 316 | 404 | n. d. | - |
| PINT | AAB58732.1 | 321 | 409 | n. d. | - |
| G_patch | CAD38742.1 | 406 | 464 | n. d. | - |
| G_patch | AAH07871.1 | 228 | 284 | n. d. | - |
| G_patch | AAH07871.1 | 233 | 284 | n. d. | +/- |
| G_patch | CAA68976.1 | 91 | 141 | n. d. | - |
| G_patch | CAA68976.1 | 91 | 146 | - | - |
| G_patch | CAA68976.1 | 96 | 141 | - | - |
| G_patch | CAA68976.1 | 96 | 146 | - | +/- |
| G_patch | AAA99715.1 | 736 | 787 | n. d. | - |
| Zpr1 | AAC33514.1 | 252 | 416 | n. d. | - |
| Zpr1 | AAC33514.1 | 257 | 416 | n. d. | - |
| Zpr1 | AAC33514.1 | 44 | 212 | n. d. | - |
| ZnF_C3H1 | AAF28981.1 | 131 | 157 | n. d. | - |
| ZnF_C3H1 | AAF28981.1 | 126 | 162 | n. d. | - |
| ZnF_C3H1 | AAF28981.1 | 131 | 162 | - | - |
| ZnF_RBZ | CAC28312.1 | 185 | 214 | n. d. | - |
| L27 | AAG34117.1 | 13 | 68 | - | - |
| L27 | AAG34117.1 | 13 | 73 | - | - |
For column '1D 1H-NMR': '-' = unfolded, '+/-' = presumably folded, '+' = folded, 'n. d' = not detectable (protein amount insufficient for detection). For column 'analytical HIC': '-' = no peak upon ammoniumsulfate gradient, but protein elutes only during GdmHCl gradient, '+/-' = very small or broad peak observed upon ammoniumsulfate gradient, '+' = distinct peak observed upon ammoniumsulfate gradient.