TABLE 2.
Relative amounts of the LH1 complexes in different culture media
| Medium and strain | Relative intensity (± 5%) of the LH1 near-IR absorption maximuma
|
|||||
|---|---|---|---|---|---|---|
| M medium
|
M2S
|
M2SF
|
||||
| A880 (or A873)b | % | A880 (or A873)b | % | A880 (or A873)b | % | |
| Semiaerobic | ||||||
| S1 | 0.14 | 100 | 0.517 | 100 | 0.77 | 100 |
| SPUHK1 | 0.13 | 93 | 0.197 | 38 | 0.64 | 83 |
| SPUHK2 | 0.10 | 71 | 0.167 | 32 | 0.79 | 103 |
| ST2 | 0.051 | 100 | 0.547 | 106 | 0.62 | 87 |
| G9 | 0.051 | 100 | 0.517 | 100 | 0.71 | 100 |
| GPUHK1 | No peak | 0 | 0.167 | 32 | 0.49 | 69 |
| GPUHK2 | No peak | 0 | 0.137 | 26 | 0.63 | 89 |
| S1 (pRKGP) | NDc | 0.517 | 100 | 0.76 | 99 | |
| SPUHK1 (pRKGP) | ND | 0.317 | 61 | 0.65 | 84 | |
| Anaerobicd | ||||||
| S1 | 0.274 | 100 | ND | ND | ||
| S1 (pRKGP) | 0.224 | 82 | ND | ND | ||
| SPUHK1 (pRKGP) | 0.214 | 78 | ND | ND | ||
a
The relative intensity of the LH1 near-IR absorption maximum was calculated from the ratio of the near-IR Qy absorption maximum (wavelength, 880 nm for S1 or 873 nm for G9/ST2) observed to the absorption maximum for the corresponding parental strain grown semiaerobically in the same medium. The relative intensity obtained for ST2 was calculated by using G9 as a reference.
b
In all cases cell suspensions were adjusted to the same A660 (turbidity) prior to measurement.
c
ND, not determined.
d
The strains were harvested in the mid-exponential phase.