TABLE 1.
PCR primers used in this study
| Primer | Sequence (5′-3′) |
|---|---|
| 1mrAE | CCGGAATTCGATTGCAATTCAGTAAGGTGG (EcoRI site is underlined) |
| 1mrAB | CGCGGATCCATGCTTTAGCTGTTGTTTCC (BamHI site is underlined) |
| MK1 | CAGTCAAAAGATCAGTCAGC |
| MK2 | TGCCGTAATACCCTTGCAGC |
| d1 | AAAAAGCCGGGTAGCTGCC |
| d2 | GATTTTCTCACGGGAATCTC |
| d3 | AACTCATAACATTCCCACCTTA |
| d4 | GCAATCAAAAATATGTGACTGG |
| d5 | CTTGATTCAATCAATCATCAATTGTCAAG |
| d3R | TAAGGTGGGAATGTTATGAGTT |
| d4R | CCAGTCACATATTTTTGATTGC |
| d5R | CTTGACAATTGATGATTGAATCAAG |
| de11 | TTCTTGACAATTGATGATTGGCAATTCAGTAAGGTGGGAA |
| de12 | TTCCCACCTTACTGAATTGCCAATCATCAATTGTCAAGAA |
| PyxaG1 | GGTGAGGAAAAAAGGGTAGC |
| PyxaG2 | CTCCGGAGCAAATAAGGCAT |
| NlmrA1 | ACGGGGCTGAACCAGATTAT |
| NlmrA2 | CAGGAATGCAGCTGGAGATA |
| NlmrB1 | GTATTCGGATTCAGCAACGC |
| NlmrB2 | ATCATCAAGCAGGTGTGCAG |
| NyxaG1 | TAGATGAAGCAAAGCCTGCA |
| NyxaG2 | AAAGCGCAAAGCCTGAGGCT |
| NyxaH1 | TGCGGCAGCAAAATCACGGT |
| NyxaH2 | CAATAGGCTGCCACGACAGA |