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Primers used to amplify afpA by PCRa
| Primer | Sequence (5′-3′)b |
|---|---|
| S3 | ATGCARCARGAYAGYCCSATYAC |
| A2 | GCIACIGGIGCRTCRAAVGT |
| B1 | TGCTGGAGGTGGTCAGAAACGTT |
| B2 | ATAGCAATGCAAGCCTGGCTGTC |
| D1c | GCCCATATGCAATACGACAGCCCAATC |
| D2d | GGATCCTAGTTACACTGCGCCGTGAAGAAC |
As shown in Fig. 1.
I, inosine; R, A or G; S, G or C; Y, C or T; V stands for G, A, or C.
The NdeI restriction site is underlined.
The BamHI restriction site is underlined.
