FIG. 6.

Deletion mapping of cspD promoter region. The scheme of the cspD locus is shown above, indicating the previously annotated coding region (solid line), and the proposed extended coding region (dashed line). The bent arrow indicates the transcription start sites determined by primer extension. Restriction sites are indicated as follows: B, BamHI; K, KpnI; No, NotI; P, PstI. The sites in parentheses were introduced by PCR and are not in the original sequence. The plasmids carrying the constructs were introduced into C. crescentus NA1000, and promoter activity was measured by β-galactosidase assays both in exponential-phase (log) and stationary-phase cells (stat). The results are in Miller units (29) and are the average of at least three independent assays, with the respective standard deviation.