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Viability of the mutant strains at 10°C. Cells of the parental strain NA1000 (○), NA1000 (ΔcspA) (•), and NA1000 (ΔcspD) (▴) were grown at 30°C up to early log phase and then transferred to 10°C. Aliquots were taken before (0 h) and 24 or 48 h after incubation at 10°C, and serial dilutions were plated to determine the number of CFU. Survival rates were determined relative to NA1000 at each time point.
