TABLE 1.
Primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| CSPA-A | CGAAACGGCTCGAGCGATG |
| CSPA-B | ATAGGATCCGTCGTTCTCAGAACCATC |
| CSPA-C | AAACTGCAGCTTCACGGTTCCAGTCGC |
| CSPA-D | TATCTGCAGCAAGCGGCCTAAAGCCCG |
| CSPA-E | AAAGGGCCCTCGCTGGTGTAGGCCTCGG |
| CSPA-PE | ATGTTCCTTGTAGGCATAGGTG |
| CSPB-A | ATAGGATCCGATCGCTCACCGCGAGAC |
| CSPB-B | GAAAAGCTTCAAGGCCTCACGCTGCGC |
| CSPB-PE | GAACCACTTTACGGTGCCG |
| CSPC-F | CGGGGTTTTTTGCGGTGCAATATCGC |
| CSPC-G | TTAGGATCCTGGCCGCCGTCCTCGGG |
| CSPC-PE | CCGTTCGCCATATTATCCTCA |
| CSPD-A | ATTGGATCCATATAACGGCTATGTTCC |
| CSPD-B | ATAGAATTCTGGTGACGATCTCGACC |
| CSPD-C | ATGGATCCACTGCCATCTTCGGC |
| CSPD-D | CCAGGATCCTGGCTTGCCCAATCAGCCC |
| CSPD-E | AAACTGCAGATTCGTCCCGAGATGATTCC |
| CSPD-F | AAAGGATCCTGGTGCGGTTCGCACGCGG |
| CSPD-G | AAAGAATTCGCTGCTCTACAGGGGTTCG |
| CSPD-PE | AAAATCGTAACCAGACATCCC |
| RT-2A | TGGTTACGATTTTGAGGACG |
| RT-2B | GTTGAACCATTTCACCTTGG |
a
Underlined nucleotides indicate restriction sites incorporated into oligonucleotides.