recU, recD, and ruvA mutations produce anucleate cells and aberrant nucleoids. (A) Exponentially growing cells were fixed, stained with DAPI, and analyzed by fluorescence microscopy to visualize the nucleoids. Black arrows indicate anucleate cells, whereas white arrows show aberrant and misplaced nucleoids. (B) Electron micrographs of cross-sectioned processed cells. The nucleoids appear as light material in the cytoplasm.