TABLE 2.

Details of oligonucleotide primers used for PCR and sequencing

Target species or primer no.a	Direction	Sequence (5′-3′)	Corresponding nucleotide positionsb
M. haemolytica and M. glucosida
471	Forward	CCAGTTGCGGTACTTCAG
472	Forward	CCAGTTGAGGTACTTCAG
156	Forward	CTCAAGCAGCTCCACAAG	50-67
158	Forward	TAGGTGCTGGTCTTGAGT	515-532
469	Forward	TTAGATGCAGCACACGCT	820-837
473	Reverse	GCTGGTTAAGGCTCTCTA
157	Reverse	GTTTGCTTCACCGTAACC	1020-1003
159	Reverse	GAGCAGCTAATTCAGGAG	559-542
468	Reverse	GTAACCACCGAATACACC	225-208
P. trehalosi
474	Forward	GTATCAGTGGCAAGCGAA
479	Forward	GTGATGGTCCAACTGCTT	494-511
485	Forward	TCGACTTTGGTAAAGCA	1061-1077
424	Forward	GGTGCTAAAGCTGGTTGG	1710-1727
482	Forward	CCAGTTGCTGAGCCAGA	2286-2302
475	Reverse	TATGCAAGCTGGCTAAGG
394	Reverse	AGCGTGTGCTGCATCTAA	2405-2388
486	Reverse	CCGTATTTACCACCGTT	1783-1767
394	Reverse	AGCGTGTGCTGCATCTAA	1122-1105
480	Reverse	TGCCACGAACACGACCAA	619-602
483	Forward	CTCGGCATAACTATCAGC
424	Forward	GGTGCTAAAGCTGGTTGG	415-432
484	Forward	TCCAGTAGCAGCTCCTGA	1002-1019
481	Reverse	GAACGCGACCGAAGTAGT	613-596

^aThe positions of the primers are shown in Fig. 1.

^bPrimer positions of internal primers for M. haemolytica and M. glucosida are based on the strain PH2 sequence (accession no. AY244653) and those for P. trehalosi are based on the sequences of strains PH68 (accession no. AY582755) and PH254 (accession no. AY582758).