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MALDI-TOF MS of 70S ribosomes from T. thermophilus strain HB8 (top) and prmA::htk null mutant TLK90 (bottom). The portions of the spectra containing proteins in the mass range of 14,000 to 18,000 Da are shown. The peaks corresponding to L11 show masses of 15,675.1 and 15,506.2 Da for the wild type and the mutant, respectively. The mass difference of 169 Da is equivalent to the mass of 12 methyl groups.
