TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strain | ||
| DH5α | φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 | 7 |
| M. xanthus strain | ||
| DK1622 | Wild type | 10 |
| DK5208 | csgA::Tn5-132 (Tcr) Ω205 | 30 |
| MDS1727 | attB::pREG1727 | This study |
| MDS5 | attB::pDS5.P (pREG1727 with 150-bp XhoI-BamHI fragment from pDS5.T)a | This study |
| MDS12 | attB::pDS12.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS12.T) | This study |
| MDS21 | attB::pDS21.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS21.T) | This study |
| MDS22 | attB::pDS22.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS22.T) | This study |
| MDS23 | attB::pDS23.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS23.T) | This study |
| MDS24 | attB::pDS24.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS24.T) | This study |
| MDS25 | attB::pDS25.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS25.T) | This study |
| MDS26 | attB::pDS26.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS26.T) | This study |
| MDS27 | attB::pDS27.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS27.T) | This study |
| MDS28 | attB::pDS28.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS28.T) | This study |
| MDS29 | attB::pDS29.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS29.T) | This study |
| MDS30 | attB::pDS30.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS30.T) | This study |
| MDS31 | attB::pDS31.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS31.T) | This study |
| MDS32 | attB::pDS32.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS32.T) | This study |
| MDS33 | attB::pDS33.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS33.T) | This study |
| MDS34 | attB::pDS34.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS34.T) | This study |
| MDS35 | attB::pDS35.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS35.T) | This study |
| MDS36 | attB::pDS36.P (pREG1727 with 235-bp XhoI-BamHI fragment from pDS36.T) | This study |
| Plasmid | ||
| pCR2.1-TOPO | Apr KmrlacZα | Invitrogen |
| pREG1727 | Apr Kmr P1-inc attP lacZ | 5 |
| pDS5.T | pCR2.1-TOPO with 150-bp fragment from −100 to +50 bp of fruA DNA generated by PCR using chromosomal DNA from M. xanthus DK1622 as template, inserted as a XhoI-BamHI fragment | This study |
| pDS12.T | pCR2.1-TOPO with 235-bp fragment from −185 to +50 bp of fruA DNA generated by PCR using chromosomal DNA from M. xanthus DK1622 as template, inserted as a XhoI-BamHI fragment | This study |
| pDS21.T | pDS12.T with CACTCCC to ACAGAAA mutation from −54 to −48 bp | This study |
| pDS22.T | pDS12.T with GCACA to TACAC mutation from −64 to −60 bp | This study |
| pDS23.T | pDS12.T with TTCGCG to GGATAT mutation from −36 to −31 bp | This study |
| pDS24.T | pDS12.T with TAGGGT to GCTTTG mutation from −13 to −8 bp | This study |
| pDS25.T | pDS12.T with GGGGCTG to TTTTAGT mutation from −44 to −38 bp | This study |
| pDS26.T | pDS12.T with TGATTCAT to GTCGGACG mutation from −72 to −65 bp | This study |
| pDS27.T | pDS12.T with CGGGCTC to ATTTAGA mutation from −79 to −73 bp | This study |
| pDS28.T | pDS12.T with C to A mutation at −61 bp | This study |
| pDS29.T | pDS12.T with C to A mutation at −54 bp | This study |
| pDS30.T | pDS12.T with A to C mutation at −53 bp | This study |
| pDS31.T | pDS12.T with C to A mutation at −52 bp | This study |
| pDS32.T | pDS12.T with T to G mutation at −51 bp | This study |
| pDS33.T | pDS12.T with C to A mutation at −50 bp | This study |
| pDS34.T | pDS12.T with C to A mutation at −49 bp | This study |
| pDS35.T | pDS12.T with C to A mutation at −48 bp | This study |
a
Where possible, the plasmid description is given in parentheses after the strain description.