FIG. 2.

Schematic location of GPS-LS insertions in the mcrA gene. The large horizontal arrow represents mcrA; some of the immediate upstream region is also shown. Vertical arrows above the gene represent amino acid insertion mutants (frames 2 and 3), and arrows below the gene represent truncation mutants (frame 1). Black arrows, RglA⁻ or McrA⁻; dashed arrows, RglAⁱ or McrAⁱ; and gray arrows, RglA⁺ or McrA⁺. The region modeled by Bujnicki and coworkers (5) (residues 159 to 272 of the gene product) is indicated. The location of the HindIII site is indicated by the H, and the insertion in mutant 1-75 is marked with an asterisk (*).