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Summary of linker mutants generated
| Expt | Transposon | No. of transformantsa | No. RglA− | No. used in final analysis |
|---|---|---|---|---|
| 1 | Transprimer-5 | 100 | 21 | 13 |
| 2 | Transprimer-5 | 100 | 25 | 13 |
| 3 | Transprimer-5 | 99 | 35 | 24 |
| 4 | Transprimer-4 | 27 | 15 | 12 |
| 5 | Transprimer-5 | 45 | 12 | 10 |
| 6 | Transprimer-5 | 99 | 21 | 12 |
| Total | 470 | 129 | 84 |
Number of transformants examined by cross-streaking for loss of RglA activity.
