TABLE 4.
Summary of previously characterized mcrA mutants
| Mutant | Phenotypea
|
Description | Reference | |
|---|---|---|---|---|
| McrA | RglA | |||
| mcrA1272::Tn10 | − | − | Tn10 insertion approx. 100 bp upstream of HindIII site | 25 |
| AP1-100-1 | +(Ts) | +(Ts) | Uncharacterized; strain derived from AP1-100 | 21 |
| AP1-100-3 | +(Ts) | i | Uncharacterized; strain derived from AP1-100 | 21 |
| MM294 ES1 | +(Ts) | i | Uncharacterized; strain derived from MM294 | 21 |
| AP1-100-9 | − | − | Uncharacterized; strain derived from AP1-100 | 21 |
| pMH611 | − | + | McrA 1-197 + 21 vector-derived residues at C terminus | 13 |
| pDRR551.6 | + | − | McrA 1-197 + 7 residues after frameshift at C terminus | 29 |
| pPA2 | + | − | 7 vector-derived residues at N terminus + McrA 14-277 | 29 |
a
Ts, temperature sensitive. These phenotypes were obtained at 30°C; restriction activity in all cases was lower at 42°C.