TABLE 1.
Plasmids used for this work
| Plasmid | Relevant features | Reference or source |
|---|---|---|
| pUC18/19 | AproriColE1 lacZα | 51 |
| pK18mob | KmroriColE1 Mob+lacZα, used for directed insertional disruption | 53 |
| pSJ3 | AproriColE1 ′lacZ promoter probe vector, lacZ fusion flanked by NotI sites | 27 |
| pUCBZDA | Apr, pUC18 containing the bzdA gene under control of the Plac promoter | This work |
| pECOR2 | Apr, pUC19 containing a 6.6-kb EcoRI DNA fragment from λBzd1 | This work |
| pECOR7 | Apr, pUC19 containing a 7.1-kb EcoRI DNA fragment from λBzd1 | This work |
| pECOR8 | Apr, pUC19 harboring a 5.4-kb EcoRI DNA fragment from Azoarcus sp. strain CIB that contains the right end of the bzd cluster | This work |
| pSJ3PR | Apr, 675-bp KpnI/XbaI bzdR fragment (PR promoter) cloned into KpnI/XbaI double-digested pSJ3 vector | This work |
| pSJ3PN | Apr, 598-bp KpnI/XbaI bzdRN fragment (PN promoter) cloned into KpnI/XbaI double-digested pSJ3 vector | This work |
| pSJ3QM | Apr, 444-bp KpnI/XbaI bzdQ-bzdM intergenic fragment cloned into KpnI/XbaI double-digested pSJ3 vector | This work |
| pSJ3VW | Apr, 313-bp KpnI/XbaI bzdV-bzdW intergenic fragment cloned into KpnI/XbaI double-digested pSJ3 vector | This work |
| pSJ3WX | Apr, 602-bp KpnI/XbaI bzdW-bzdX intergenic fragment cloned into KpnI/XbaI double-digested pSJ3 vector | This work |
| pSJ3YZ | Apr, 277-bp KpnI/XbaI bzdY-bzdZ intergenic fragment cloned into KpnI/XbaI double-digested pSJ3 vector | This work |
| pK18mobPNlacZ | Kmr, EcoRI/HindIII PN::lacZ fragment from pSJ3PN cloned into EcoRI/HindIII double-digested pK18mob vector | This work |
| pK18mobbzdN | Kmr, 523-bp EcoRI bzdN internal fragment cloned into EcoRI-digested pK18mob | This work |
| pK18mobbzdY | Kmr, 366-bp EcoRI bzdY internal fragment cloned into EcoRI-digested pK18mob | This work |