TABLE 2.

List of primers used in this work for PCR and RT-PCR reactions

Primer no.	Primer name	Sequence (5′-3′)a	Amplified fragmentb
1	NIN35	GGAGTCGGTGGTCGCAAGC	313-bp bzdR internal fragment
2	REG	GTGCCAGGACTTCGAGGATGTC
3	BzdN5′	CGAATTCTGATCGAGGCCGGCATGCTG (EcoRI)	523-bp bzdN internal fragment cloned in pK18mobbzdN
4	BzdN3′	CGAATTCAGCATCTCGTTGTGCTC (EcoRI)
5	BzdP5′	CCAAGGAGCCGAGCTTCGC	399-bp bzdP internal fragment
6	BzdP3′	CCGAATTCCTTGAGCGACAGC
7	δBcr5′	GCTACGGCCGGGTGAACGTGC	487-bp bzdQ internal fragment, bzdQ probe
8	δBcr3′	CTTCACGACGCCCGGGTTCTTC
9	BzdQM5′	GCGGCGCTGTTCGGCTAC	217-bp bzdQM intergenic region
10	BzdQM3′	GATGCTGATGTAATACTCGCCGG
11	EcoR8	GGCGTCGATCCGCGCTTCC	503-bp bzdTU intergenic region
12	BzdU3′	CATCGGGAGGTAGTTGTCGCG
13	EcoR11	CTGATCGAATCCGGCTGTAC	523-bp bzdV internal fragment
14	BzdV4-11	GGAACGCGGTAGGACGGG
15	BzdVW5′	GGAACAGCTCGAACGTGGCGG	515-bp bzdVW intergenic region
16	BzdVW3′	GGGCGACGTTCATCTCCTCCATC
17	BzdWXII5	GCACCCGCAAGACGATCCG	544-bp bzdWX intergenic region
18	BzdWXII3	GCCGATCACTGTGCCGGAG
19	BzdY5′	CGAATTCAGTACAACTCCTACACCACC (EcoRI)	366-bp bzdY internal fragment cloned in pK18mobbzdY, bzdY probe
20	BzdY3′	GGAATTCTGCCGTGCTTCGGGCCGGC (EcoRI)
21	YZ5′	CGGCAAGGACGTCATCGACTTC	322-bp bzdYZ intergenic region
22	YZ3′	CCGCCGCGCCGATGCCCTG
23	BzdZ-15′	GTTGAAGGACAGGGTCGCAATCG	447-bp bzdZ internal fragment
24	8.3.4.	CTGCCCGATCGTGCCGAGC
25	BzdA-25′	GGGCACGCGCGGTGGACATC	577-bp bzdA internal fragment
26	8.3.2.	CCCCTGCCCTGTTCGAGAG
27	3REG	GGGGTACCCGTGCATCAATGATCCGGCAAG (KpnI)	490-bp bzdRN intergenic region
28	N-INV-2	GCAGTGCAGGCGATGTTGAT
29	5PBM	GGGGTACCGAAGTCCGAAGCGCTGGGTCTGC (KpnI)	444-bp bzdQM fragment cloned in pSJ3QM
30	3PBM	GCTCTAGACCCATTTTTTCCCTCCTCGGGCACTTAGTAGG (XbaI)
31	5PBZDX	GGGGTACCACATGCTCGTCGCTGCCTCCAAC (KpnI)	602-bp bzdWX fragment cloned in pSJ3WX
32	3PBZDX	GCTCTAGACCCACGCTTCGTCTCCCTTTAGCTTTCGG (XbaI)
33	5PBZDYZ	GGGGTACCGGCAAGGACGTCATCGACTTC (KpnI)	277-bp bzdYZ fragment cloned in pSJ3YZ
34	3PBZDYZ	GCTCTAGACCCATGCAAGTCCCCTTTAACCGC (XbaI)
35	RIVW5′	GCGGTACCTGCAGCAGTACGGAAAGATG (KpnI)	313-bp bzdVW fragment cloned in pSJ3VW
36	RIVW3′	GCTCTAGACCCATGGTGTCTCCCCTGGTTC (XbaI)
37	3REG	GGGGTACCCGTGCATCAATGATCCGGCAAG (KpnI)	598-bp bzdRN fragment (PN) cloned in pSJ3PN
38	5BZN	GCTCTAGACCCATCGAACTATCTCCTCTGATG (XbaI)
39	5REG	GGGGTACCGGTTTCGTCGCAGGTGCTGTCTGGC (KpnI)	675-bp bzdR fragment (PR) cloned in pSJ3PR
40	3PREG	GCTCTAGACCCATCAGCAGGTAGTTGTTCTCTTAACG (XbaI)
41	AINI	CCGAATTCTGAATAGATAAGGAGAGGAGGAGCAAATGGCAGAAC (EcoRI)	1,913-bp bzdA gene cloned in pUCBZDA
42	BZDB′3′	CGCCGAACGAGTATTTCCAGCTC
43	1600R	AAGGAGGTGATCCAGCC	1,525-bp fragment of the 16S rDNA genes
44	16SF1	GAGASTTTGATCHTGGCTCAG

^aEngineered restriction sites are underlined, and the corresponding restriction enzyme is shown in parentheses.

^bPCR reactions were carried out with the sets of two primers indicated to the left.