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Purification of PvdH from P. aeruginosa PAO1
| Step | Protein (mg) | Activity (U)a | Sp act (U/mg) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extract | 796 | 1.42 | 0.01 | 100 | |
| Source 15Q | 44 | 0.2 | 1.72 | 14 | 172.2 |
| Superdex 200 | 26 | 0.09 | 2.21 | 1 | 220.7 |
One unit was the amount of enzyme required to oxidize 1 μmol of NAD(P)H/min at pH 7.5
