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. 2004 Sep;186(17):5926–5932. doi: 10.1128/JB.186.17.5926-5932.2004

TABLE 2.

B. subtilis strains and plasmids used in this study

Strain or plasmid Relevant characteristics Reference or source
B. subtilis strains
    DB2 trpC2 ezrA (wild type) B. subtilis 168
    DB430 trpC2 npr apr epr bfp isp1 18
    DB2001 trpC2 ezrA (ΔP1) Cmr This study
    DB2002 trpC2 ezrA (ΔP2) Cmr This study
    DB2003 trpC2 ezrA (ΔP1P2) Cmr This study
Plasmids
    pET21d E. coli protein overexpression vector, Ampr Novagen Inc.
    pKM1 Derivative of pET21d, containing an expressible ezrA from B. subtilis DB2 This study
    pCT20 pCT18-derived plasmid which contains P1P2, P7, and P8 promoters of sigA operon, followed by the T1T2 terminators of E. coli rrnB operon 28
    pKM3 pCT20-derived plasmid containing DNA fragment encompassing the 500-bp of DNA upstream of ezrA followed by T1T2 terminators of E. coli rrnB operon This study
    pWP18 Promoter probing vector with aprE reporter 34
    pEZ1 to -6 pWP18 derivative containing putative promoter DNA fragments with different length This study
    pCioZA Integration vector with lacZ as reporter; Ampr Ermr 34
    pEZ8, -10 to -13 pCoiZA derivative containing different putative promoter DNA fragments and used for construction of B. subtilis DB22, DB21, DB23, DB24, DB25, respectively, by integration into the aprE locus on B. subtilis DB2 chromosome This study
    pDP1 Integration vector used for construction of B. subtilis DB2001 This study
    pDP2 Integration vector used for construction of B. subtilis DB2002 This study
    pDP12 Integration vector used for construction of B. subtilis DB2003 This study