B. subtilis strains |
|
|
DB2 |
trpC2 ezrA (wild type) |
B. subtilis 168 |
DB430 |
trpC2 npr apr epr bfp isp1 |
18 |
DB2001 |
trpC2 ezrA (ΔP1) Cmr
|
This study |
DB2002 |
trpC2 ezrA (ΔP2) Cmr
|
This study |
DB2003 |
trpC2 ezrA (ΔP1P2) Cmr
|
This study |
Plasmids |
|
|
pET21d |
E. coli protein overexpression vector, Ampr
|
Novagen Inc. |
pKM1 |
Derivative of pET21d, containing an expressible ezrA from B. subtilis DB2 |
This study |
pCT20 |
pCT18-derived plasmid which contains P1P2, P7, and P8 promoters of sigA operon, followed by the T1T2 terminators of E. coli rrnB operon |
28 |
pKM3 |
pCT20-derived plasmid containing DNA fragment encompassing the 500-bp of DNA upstream of ezrA followed by T1T2 terminators of E. coli rrnB operon |
This study |
pWP18 |
Promoter probing vector with aprE reporter |
34 |
pEZ1 to -6 |
pWP18 derivative containing putative promoter DNA fragments with different length |
This study |
pCioZA |
Integration vector with lacZ as reporter; Ampr Ermr
|
34 |
pEZ8, -10 to -13 |
pCoiZA derivative containing different putative promoter DNA fragments and used for construction of B. subtilis DB22, DB21, DB23, DB24, DB25, respectively, by integration into the aprE locus on B. subtilis DB2 chromosome |
This study |
pDP1 |
Integration vector used for construction of B. subtilis DB2001 |
This study |
pDP2 |
Integration vector used for construction of B. subtilis DB2002 |
This study |
pDP12 |
Integration vector used for construction of B. subtilis DB2003 |
This study |