FIG. 1.

Biofilm formation by B. bronchiseptica grown in the Bvgⁱ phase. Overnight liquid cultures of B. bronchiseptica were grown in the Bvgⁱ phase (0.8 mM nicotinic acid, left) or Bvg⁻ phase (4 mM nicotinic acid, right) in polystyrene culture tubes in continuous rotation on roller drums. In the Bvgⁱ phase, a majority of the bacteria were adherent to the test tube wall, in contrast to bacteria that was grown in Bvg⁻ phase (or Bvg⁺ phase; data not shown) in which most bacterial cells remained in the liquid media.