Table 1. The sequences of specific primers for real-time PCR operation.
| Gene | Prime sequence (5′→3′) | Product size/bp | Annealing temperature/°C |
|---|---|---|---|
| Col I | Forward: GGTGATGCTGGTCCTGTTG | 121 | 60 |
| Reverse: CATCGTGAGCCTTCTCTTGAG | |||
| Col VI | Forward: GCTGGAGGATGCTGTGAAC | 103 | 60 |
| Reverse: CGTGGCGATGATGCTCAG | |||
| SCX | Forward: AGAACACCCAGCCCAAACA | 64 | 60 |
| Reverse: TCCTTGCTCAACTTTCTCTGGT | |||
| TNC | Forward: ACCGTCTCTTCCGTCACTTCT | 122 | 60 |
| Reverse: AACAACTTAGGACAATGCGTCT | |||
| TNMD | Forward: TCACGCCAGACAAGCAAGT | 192 | 60 |
| Reverse: CGTCCTCCTTGGTAGCAGTATG | |||
| EYA2 | Forward: GTGTGGAAGAGGAGCAAGGA | 143 | 60 |
| Reverse: GTGAGATGGCAGGTGGAGAT | |||
| β-Actin | Forward: GGACCTGACTGACTACCTCAT | 107 | 60 |
| Reverse: CGTAGCACAGCTTCTCCTTAAT |
EYA2, eye absent 2; PCR, polymerase chain reaction; SCX, scleraxis; TNC, tenascin-C; TNMD, tenomodulin.