Table 1. The sequences of the gene-specific primers used for the real-time qPCR analysis.

Gene	Forward primer	Reverse primer	Size/bp
Mouse/rat Amel	5′-GATGGCTGCACCACCAAATC-3′	5′-CTGAAGGGTGTGACTCGGG-3	65
Mouse Enam	5′-TGCAGAAATCCGACTTCTCCT-3′	5′-CATCTGGAATGGCATGGCA-3′	114
Rat Enam	5′-ACCGAAAGTCCTGACACATTGG-3′	5′-CCTGAAGCAGTAAACAGCCGTG -3′	154
Mouse/rat Mmp-20	5′-GGCGAGATGGTGGCAAGAG-3′	5′-CTGGGAAGAGGCGGTAGTT-3′	166
Mouse Ambn	5′-ATGAAGGGCCTGATCCTGTTC-3′	5′-GTCTCATTGTCTCAAGGCTCAAA-3′	130
Mouse/rat Epfn	5′-CCTTTCTATTTCACCCTCCCCTG-3′	5′-CCACCACCTCATCTCTGCTTTCTC-3′	282/248
Rat Runx2	5′-CGGGAATGATGAGAACTACTC-3′	5′-TGAAACTCTTGCCTCGTCC-3′	118
Mouse/rat GAPDH	5′-CCATCACCATCTTCCAGGAG -3′	5′-GCATGGACTGTGGTCATGAG-3′	322

Ambn, ameloblastin; Amel, amelogenin; Enam, enamelin; Epfn, epiprofin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Mmp, matrix metalloproteinase; qPCR, quantitative polymerase chain reaction; Runx2, Runt-related transcription factor 2.

The real-time qPCR analysis was performed using a StepOne kit with SYBR green PCR reagent with the listed primers.