TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Genotype or description	Source (reference)a
Strains
B. subtilis
PS832	Prototroph, wild-type	Laboratory stock
CVO1724b	gerQ-gfpc	33
FB111	ΔcwlJ::tet	24
FB112	ΔsleB::spc	24
KB29	ΔgerQc::spc	33
KB81	Δtgl::ermC	pKE80→PS832
KB82	ΔsleB::spc Δtgl::ermC	KB81→FB112
KB83	gerQ-gfpc Δtgl::ermC	CVO1724→KB81
KB101	Δtgl::cat	pPS2742→KB81
KB102	cwlJ-His tag Δtgl::cat	PS3449→KB101
KB104	ΔspoIVA::spc	RL2037→PS832
PS2495	sodA::cat	4
PS3328	ΔcotE::tet	24
PS3449	cwlJ-His tag	3
RL2037	ΔspoIVA::spc	32
E. coli
KE96	BL21 λ(DE3) pLysS pET11a-gerQc	pKE95→PS1404
KE98	BL21 λ(DE3) pLysS pET16b-gerQc	pKE97→PS1404
PS1404	BL21 λ(DE3) pLysS	37
PS2602	BL21 λ(DE3) pLysS pET11a	30
Plasmids
pET11a	Protein expression vector	Novagen
pET16b	N-terminal His10 tag fusion protein expression vector	Novagen
pKE80	Δtgl::ermC	This work
pKE95	pET11a-gerQc	This work
pKE97	pET16b-gerQc	This work
pPS2742	ΔermC::cat	35

^aDNA from the strain or plasmid to the left of the arrow was used to transform the strain to the right of the arrow.

^bGenetic background is PY79 (prototroph, wild type).

^cgerQ is the new name for the ywdL gene identified as described in reference 33.