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Oligonucleotide primers used for the amplification of ptx junction sequences
| Amplified junction | Primer set (5′-3′) | Predicted product size (bp) |
|---|---|---|
| ptxAB | ATGAGCCGGTAGCCAGTCT, | 561 |
| AAATACGCCAGGTCGATACG | ||
| ptxBC | GGGCAGGACTACGAACAACA, | 611 |
| TCGATAGCCCGAAAAGTCTG | ||
| ptxCD | CATGGTCGGCAAGTTCTTC, | 563 |
| CCGACTACACGCAGCTCA | ||
| ptxDE | GAGCTGCTTGCCCTCGTA, | 598 |
| CCATGCAGGGCTTCTAGC |
