FIG. 1.

Organization of the A. cellulolyticus cellulosome-integrating cluster. (A) Arrangement of the four scaffoldin genes, bordered upstream by a putative cellobiose phosphorylase gene (cep) and downstream by a putative nucleotide methylase gene (nme). Immediately downstream of scaA, scaC, and scaD are located putative transcription terminators (hairpin loops), designated by lollipop-like symbols. (B) The scaD gene was sequenced in this work. The scaD gene encodes a protein containing a signal peptide (crosshatched), three cohesins (numbered), and a C-terminal SLH module. The modules of ScaD are all separated by well-defined Pro/Thr-rich linker segments (black boxes).