FIG. 4.

Affinity blotting of selected dockerin-containing fusion proteins with the two types of recombinant ScaD cohesins. Recombinant fusion proteins, comprising Geobacillus stearothermophilus xylanase T6 and C-terminal dockerins from ScaA (DocA), ScaB (DocB), or the cellulosomal GH9B enzyme (DocGH9), were expressed in an appropriate E. coli host cell system. The ∼50-kDa Xyn-Doc fusion proteins were subjected to SDS-PAGE (Gel), transferred to nitrocellulose membranes (Blots), and probed with the ScaD cohesins (CohD1 and CohD3, respectively) as described in the legend to Fig. 3.