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. 2004 Sep;186(17):5672–5684. doi: 10.1128/JB.186.17.5672-5684.2004

FIG. 5.

FIG. 5.

Expression of fimT, fimU, pilV, pilW, pilX, pilY1, pilY2, and pilE in trans complemented the twitching motility defect in the algR mutant PSL317. (A) Representative photographs of the twitching motility of the wild-type strain PAO1, the algR mutant PSL317 (ΔalgR), and PSL317 containing the complementation plasmid pVDtacPIL showing the zone of twitching motility. (B) Quantified twitching motility zones for PAO1, PSL317, and PSL317(pVDtacPIL) (n = 5 for all strains). No twitching motility was observed for PSL317 on any replicate. Error bars represent standard errors. **, P < 0.01; ***, P < 0.0001. (C) Western blot analysis of AlgR production in PAO1 (lane 1), PSL317 (lane 2), and PSL317(pVDtacPIL) (lane 3). Equivalent amounts of total protein (25 μg) were separated on a 4 to 20% gradient sodium dodecyl sulfate-polyacrylamide gel. The proteins were blotted and probed with an anti-AlgR monoclonal antibody (see Materials and Methods). MW, molecular weight standards (weights are given at left in thousands).