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The piRNA biogenesis and silencing pathway. dN/dS estimates, indicated with varying intensity of red, between D. melanogaster and D. simulans piRNA pathway components were obtained from the flyDIVaS web server [81], with the exception of estimates for ago3, deadlock, Yb, maelstrom, rhino, squash, vasa, and valois. dN/dS estimates for these missing genes were obtained by through our analysis.
