Fig. 3.

Flow cytometric analysis of representative tissue from the inguinal lymph node. a The CD19/CD5 plot demonstrates and admixture of CD19-positive B cells and CD5-positive T cells. b Gating on the CD19 positive B cells demonstrates a clear lambda bias. c the lambda bias of is also seen when gating on CD20 positive B cells. d The majority of the CD19 positive B cells were found to be CD20 positive and CD10 negative. The presence of a subset of CD10 positive B cells and subset positivity for kappa light chain staining is compatible with germinal center B cells that are admixed with the malignant B cells