Fig. 2.

Target cccDNA generated from virion associated rcDNA. a PCR analysis of the serum of 4 HBV infected patients (S1–S4) in presence or absence of DNase I treatment. TP53 amplification (top) and HBV (bottom). b Sequences of HBV from the serum of the 4 infected patients. The sequence from serum 1 was used as reference, the number of sequences analysed is given on the right. Above is a representation of rcDNA for this region. c Quantification by real-time PCR based on SYBR Green on serum S1. d SYBR Green PCR quantification after DNase treatment followed by Plasmid-safe DNase and T4 exonuclease on serum S1, S2 and S3. All amplifications were controlled by gel electrophoresis. Above a Ct of 32.3 only primer-dimers were recovered