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Chromatogram illustrating (A) normal resolution of disialo and trisialotransferrin (arrow) in a non-cirrhotic heavy drinker and (B) di–tri bridging (arrow) in a cirrhotic subject followed by tetra- and penta-sialo transferrin peaks. The y-axis represents absorbance of iron-saturated transferrin at 470 nm and the x-axis represents elution time in minutes. Di–tri bridging may be seen in cirrhotic or non-cirrhotic severe liver disease and when heavy alcohol use may or may not be present.
