Fig. 2.

Biotinylation analysis of constitutive hOAT4 recycling. a. 30-min recycling of transferrin receptor (TfR) was analyzed as described in “Materials and Methods” section followed by Western blotting using anti-TfR antibody (1:100). b. hOAT4 recycling was analyzed at a 5-min time point followed by Western blotting using anti-myc antibody (1:100). c. Densitometry plot of results from Fig. 2b as well as from other experiments. Total biotin-labeled hOAT4 was expressed as % of hOAT4 biotinylated at 4°C (before initiation of recycling). Values are mean ± S.E. (n=3).