FIGURE 1:

IFT20 protein expression in the wild-type and conditional mutant mice. (A) Analysis of tissue distribution of mouse IFT20 protein by Western blot using a high-sensitivity Femto system. Note that IFT20 is highly expressed in the testis and is also present in most somatic tissues. (B) IFT20 expression levels during the first wave of spermatogenesis. Its expression is significantly increased at day 16 of birth and further increased at day 30 of birth, when germ cells enter spermiogenesis. (C) Examination of IFT20 protein expression by immunofluorescence staining on testicular sections (left) and isolated germ cells (right). Strong IFT20 signals (red) are observed in the control samples (top). However, almost no specific IFT20 signals are observed in the knockout samples (bottom). (D) Analysis of testicular IFT20 expression by Western blot using the Pico system. IFT20 was expressed in the three control mice. However, no specific signal was observed in the three mutant mice analyzed.