FIGURE 2:

Nonciliary Arl13b^V358A mutant is defective in Shh-stimulated MEF migration despite Arl13b^V358A being biochemically indistinguishable from wild-type Arl13b protein. (A) Average total migration for Arl13b^hnn MEFs expressing Arl13b-GFP mutants, normalized to wild-type Arl13b-GFP, migrating toward rShhN. N = 12. Analysis described in Materials and Methods. (B) Recombinant mouse Arl13b and Arl13b^V358A display indistinguishable intrinsic and GAP-stimulated rates of GTP hydrolysis. (C) Arl13b^hnn MEFs expressing GFP or Arl13b^WT-GFP, normalized to the GFP-only control, migrate similarly toward fetal calf serum (FCS). (D) Arl13b^hnn MEFs expressing GFP show impaired migration toward the Smo agonist purmorphamine, which can be rescued by Arl13b^WT-GFP.