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. 2016 Dec 1;27(24):3800–3812. doi: 10.1091/mbc.E16-04-0224

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© 2016 Génier et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — CCT7 coimmunoprecipitates with other GPCRs. (A) Lysates of HEK 293 cells transiently expressing HA-MOR (HA-tagged rat μ-opioid receptor) alone or with CCT7-MYC were immunoprecipitated with an HA-specific monoclonal antibody and analyzed by immunoblotting with MYC- and HA-specific HRP-conjugated antibodies. Lysates of HEK 293 cells transiently expressing FLAG-DOR (FLAG-tagged rat δ-opioid receptor; B) or FLAG-DP (FLAG-tagged prostaglandin D₂ receptor; C) alone or with CCT7-MYC were immunoprecipitated with a FLAG-specific monoclonal antibody and analyzed by immunoblotting with FLAG-specific polyclonal and HA-specific HRP-conjugated antibodies. The blots shown are representative of three separate experiments. IB, immunoblotting; IP, immunoprecipitation.