FIGURE 2:

ACTH-induced expression of TIS11b mRNA in adrenocortical cells is transcription dependent. (A) Time-course analysis of TIS11 family member expression in BAC cells challenged with ACTH. RT-qPCR was performed as described in the Supplemental Material. Expression levels of TTP, TIS11b, and TIS11d mRNAs were normalized to RPL27 mRNA. Values are means ± SD of four independent experiments. (B) Northern blot analysis of TIS11b transcript levels in BAC cells challenged with ACTH in the absence or presence of the transcription inhibitor DRB. The membrane was hybridized to a radiolabeled TIS11b cDNA probe and rehybridized to 18S RNA probe for loading control. Shown is a representative Northern blot of six independent experiments.