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. 2016 Dec 1;27(24):3883–3893. doi: 10.1091/mbc.E16-08-0586

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© 2016 Tanaka, Ono, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Subcellular localization of ATP9A. HeLa cells stably expressing C-terminally HA-tagged ATP9A were doubly stained for HA and organelle markers EEA1 (early endosome), TfnR (early/recycling endosome), Lamp-1 (late endosome), TGN46 (TGN), and GM130 (cis-Golgi), followed by Cy3-conjugated anti-rat and Alexa Fluor 488–conjugated anti-mouse or anti-rabbit secondary antibodies. The recombinant EGFP-Lactadherin C2 domain (rEGFP-LactC2) was treated with anti-HA antibody after permeabilization of cells with digitonin. Images were obtained by confocal microscopy. Insets are enlarged in the right panels. Scale bars: 10 μm.