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. 2016 Dec 15;11:6561–6574. doi: 10.2147/IJN.S117745

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© 2016 Orlov et al. This work is published and licensed by Dove Medical Press Limited

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Survival of Escherichia coli cells expressing the fusion protein GST-NdCTR1 or GST depends on the time of treatment with silver nanoparticles.

Notes: (A) Protocols of the experiments demonstrating that NdCTR1 rescued bacteria treated with the SNPs. (B and C) Histograms showing survival of E. coli cells expressing GST (black) or GST-NdCTR1 (gray) after treatment with 10 μM SNPs and 100 μM SNPs.

Abbreviations: SNPs, silver nanoparticles; NdCTR1, N-terminal domain of CTR1; GST-NdCTR1, glutathione-S-transferase-N-terminal domain of CTR1; BL21 (DE3)/pGEX-4T-1, E. coli strain synthetizing GST; BL21 (DE3)/pNdCTR1, E. coli strain synthetizing GST-NdCTR1; CFU, colony-forming unit.