Table 1.

List of sgRNAs designed to target grape MLO-7 gene locus and apple DIPM-1, 2, and 4 gene loci.

Target	sgRNA	Sequence (5′-3′)
MLO-7	RG1	TATGCATTTCTGAGAGTGTTGGG
	RG2	CACTTGGCACCCTTGTAAAAAGG
	RG3	CCAAAGATTTTAAGAACACATGC
	RG4	TTTTAAGAACACATGCTCTGAGG
DIPM-1	RG1	CGTCGTTTCAGCTCAACCCGGGG
	RG2	CGATTGGCTGGTGAGGTAATTGG
	RG3	AAGCACAGATCCCAGAACGGAGG
	RG4	GATCCCAGAACGGAGGGAGGTGG
	RG5	CGAGAGCAATTCCAAGGAGCGGG
	RG6	TAGCATTGGGACTACGTACAAGG
	RG7	CATGGATGAGGGACGACGATTGG
	RG8	TACTGGACTTGCGAATCTCGGGG
DIPM-2	RG1	ACGGAAGAGAGAGGTTGCGGAGG
	RG2	CAGTTTCTAGAAGCTTCGAGCGG
	RG3	CTCGTACCCGGGTTGGGCAGAGG
	RG4	GGTCGGATGGTGTTCTTCGAAGG
	RG5	CCCGCCTTTCCCCAGCATCTCGG
	RG6	CACGGGGAAGTGTCCCTCCGTGG
DIPM-4	RG1	AGCGACTACGCGGTCTTTATTGG
	RG2	GAACAAGCCGGCAAGGGGCTCGG
	RG3	ACGATCGCAACGATTGCGCCGGG
	RG4	AAGGTGTACGCGAACAGCGGGGG
	RG5	ACGGTACAAACGCGACGGACAGG
	RG6	GGGGAAAGGAAGCCTAGGGACGG
	RG7	GCTGTATTCCGCATGAATCCTGG

Sequences of sgRNA in bold indicate selected sgRNAs used in the study for transformation. Underlined nucleotides indicate PAM motifs in sgRNA sequences.