Table 2.

List of primers used for in vitro cleavage assay and targeted deep sequencing.

Target	Sequence (5′-3′)
MLO-7 in vitro cleavage assay	F; GCAGTGGTTAAAAGGCAGAC R; CTTGGTTCTTCCCAAAGCC
MLO-7 deep sequencing	F; CCAAAGGTCTAACCCTTTTC R; GGGAAACACCTTTTTCAGTC
DIPM-1 in vitro cleavage assay	For RG1 – RG6 F; TTTTAATCTTCAACGTCTCC R; TTGCCTGAAAATAAGCCCTC
	For RG7 and RG8 F; TAGTAACCAAAGGGAAGTGG R; TTCAACACTTGCCACATTGC
DIPM-1 deep sequencing	F; GTCTTATGCCTCTTTGCGG R; CTCCAGACTGTATAGCTGAG
DIPM-2 in vitro cleavage assay	For RG1 F; TGCACAAATAACCGAGTCTC R; ACTATGATGGCGATTAGAGC
	For RG2 to RG5 F; CAAGGGTACATCAAACGACC R; AAATTTACCGTGGAGAACCC
	For RG6 F; ACATCCTCTTAGACAAGACC R; AGCACAAACGAAAACGAGAG
DIPM-2 deep sequencing	F; CGCTGCTCCTGTACTGCTAC R; GTCGAGCACCGCCTTGTA
DIPM-4 in vitro cleavage assay	For RG1 F; AGAAAAACAAGCCTTTCGCG R; TCCGTACAATTCGTTGTTGG
	For RG2 to RG6 F; CACCAACAACGAATTGTACGG R; AGTAATAAGCACTCAGCCTC
	For RG7 F; TGCAGTTTGAGTCTAATGCG R; CCTCAATGTTCTTGTACCTC
DIPM-4 deep sequencing	F; GATGTAATTAAGGGAATCGG R; CAATCTTGCAATGGCGTGAA