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. 2016 Nov 1;27(21):3214–3220. doi: 10.1091/mbc.E16-07-0479

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© 2016 Tucker et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.(http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — SN dopamine neurons display high autofluorescence. (A) Representative single-plane multiphoton images of SN pars compacta (SNc), substantia nigra pars reticulata (SNr), hippocampus (Hipp), and cortex (Cort) at matched depth and laser power. (B) Pseudocolored summed z-projection of a sulforhodamine B (magenta)–filled SN neuron. The insets are single-plane pseudocolored magnifications of autofluorescence (yellow; left) and sulforhodamine B/autofluorescence overlay (magenta/yellow; overlap indicated by white) in individual dendrites and the soma. (C) Somas of the SN with high autofluorescence exhibit electrophysiological characteristics of DA neurons. Representative single-plane image (C1) and current-clamp voltage traces of slow pacemaker activity (C2), broad action potentials (C3), and Ih voltage sag (held at −60 mV, followed by a 2-s, −250 pA current injection; C4) characteristic of DA neurons. (D) Representative single-plane pseudocolored images of autofluorescence (yellow) colocalization with monoamine neurons identified with IDT307 (fluorescent monoamine transporter substrate; magenta) in the SN. Scale bars, 10 μm (A–D), 5 μm (insets).