FIGURE 7:

P67 preferentially binds to CDK5/p35 in the presence of TFP5 in cotransfected HEK 293 cells. HEK 293 cells were cotransfected with Cdk5/p35 (myc-his)/p67 or Cdk5/p25 (myc-his)/p67 and incubated in the presence of different concentrations of TFP5 peptide for 24 h. Lysates were then prepared and subjected to Western blotting to visualize protein expression of transfected plasmids with respective antibodies. (A) Expression of different proteins in the lysates using p67, p35/p25, Cdk5, and β-actin antibodies. (B) Immunoprecipitation using 500 μg of proteins (p35 myc-his and p25 myc-his) with monoclonal his-tag antibody detected with p67, p35, Cdk5, and β-actin antibodies, respectively, after Western blotting. (C) Quantification of immunoblots of p67 and p35 antibodies, respectively, from three experiments (± SEM). **p < 0.01.