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. 2016 Nov 1;27(21):3329–3341. doi: 10.1091/mbc.E16-03-0184

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© 2016 Toft-Bertelsen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — SNAP-25 WT and KO cells show similar relative expression of calcium channel subtypes. (A) Representative traces from a single-KO adrenal chromaffin cell during sequential additive application of calcium channel blockers. Bottom to top: nifedipine (L type, 3 μM), ω-conotoxin (N type, 1 μM), agatoxin (P/Q type, 1 μM), SNX482 (R type, 300 nM), Ni²⁺ (T type, 40 μM). (B) Sequential application of blockers did not reveal large differences in the fraction of calcium channel subtypes between SNAP-25 WT and KO cells. N = 3, n = 10–13.