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. 2016 Nov 1;27(21):3357–3368. doi: 10.1091/mbc.E16-07-0506

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© 2016 Chen et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Fixation does not seriously perturb nuclear structure. (A) A tomographic slice (10 nm thick) of an unfixed US1363 G1-arrested cell. (B) A 15-fold enlargement of the nuclear densities boxed in A. (C) A tomographic slice (10 nm thick) of a formaldehyde-fixed US1363 cell. (D) A 15-fold enlargement of the nuclear densities boxed in in C. Arrows point to the mitochondrial membranes. Scale bars, 200 nm.