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. 2016 Dec 19;9:648. doi: 10.1186/s13071-016-1928-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Antibody titres of IgG and isotopes triggered by rCsCP and coat proteins of B.s-CotC-CsCP spores via subcutaneous immunization route. ELISA evaluation of the CsCP specific IgG a and IgG1/IgG2a c levels in mouse sera after subcutaneous immunization with rCsCP. b Antibody titres of IgG induced by rCsCP at week 6. The levels of CsCP specific IgG d and IgG1/IgG2a f in the sera of mice subcutaneously immunized with spore coat proteins of B.s-CotC-CsCP. Antibody titres of IgG evoked by spore coat proteins of at week 6 were also assayed by ELISA e. Data were displayed as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Abbreviations: B.s-CotC-CP, WB600 containing pEB03-CotC-CsCP; B.s-CotC, WB600 containing pEB03-CotC; rCP, purified rCsCP